N-Tigloylglycine is primarily utilized in biochemical research as a metabolite for studying metabolic pathways and disorders. It plays a role in understanding the metabolism of certain amino acids and fatty acids, particularly in the context of inborn errors of metabolism. Researchers use it to investigate the biochemical processes involved in conditions like tiglic acidemia, where its accumulation can provide insights into enzymatic deficiencies. Additionally, it serves as a reference compound in analytical techniques such as mass spectrometry and chromatography to identify and quantify similar metabolites in biological samples. Its application extends to the development of diagnostic tools for metabolic diseases, aiding in the early detection and monitoring of such conditions.