8-Hydroxyguanosine is widely utilized as a biomarker for oxidative stress and RNA damage in biological systems. It is particularly significant in studies related to aging, cancer, and neurodegenerative diseases, where oxidative damage to RNA is a critical factor. Researchers measure its levels in tissues, blood, or urine to assess the extent of oxidative damage and the effectiveness of antioxidants or therapeutic interventions. In clinical research, it helps in understanding the role of oxidative stress in disease progression and in developing strategies to mitigate its effects. Additionally, it is used in environmental toxicology to evaluate the impact of pollutants or radiation on cellular RNA integrity. Its detection and quantification provide valuable insights into cellular health and the mechanisms underlying oxidative damage.