Used in biochemical research to study vitamin B6 metabolism, as it is a major metabolite of pyridoxine. It helps in understanding the role of vitamin B6 in enzymatic processes and its impact on human health. Additionally, it serves as a biomarker in clinical diagnostics to assess vitamin B6 status and deficiencies. In pharmaceuticals, it aids in developing treatments for conditions related to vitamin B6 imbalance. Its applications also extend to nutritional science, where it supports research on dietary requirements and supplementation strategies.