MMP-9-IN-1 is primarily utilized in research settings to study the role of matrix metalloproteinase-9 (MMP-9) in various biological processes. It is applied as a selective inhibitor to investigate the enzyme's involvement in tissue remodeling, inflammatory diseases, and cancer progression. Researchers use it to explore MMP-9's impact on extracellular matrix degradation, cell migration, and angiogenesis. Additionally, it aids in understanding the potential therapeutic benefits of targeting MMP-9 in conditions like arthritis, cardiovascular diseases, and tumor metastasis. Its application is crucial for developing novel treatments by modulating MMP-9 activity in pathological states.