Alpha-Cyano-3-hydroxycinnamic Acid is widely used in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry as a matrix compound. It facilitates the ionization of analytes, particularly peptides, proteins, and other biomolecules, enabling their detection and analysis. This chemical is especially effective for studying low molecular weight compounds due to its ability to produce clear and distinct mass spectra. Additionally, it is utilized in the analysis of polymers, synthetic organic molecules, and small molecules in various research and industrial applications. Its role in MALDI imaging also aids in the spatial mapping of molecules in biological tissues, contributing to advancements in medical and biological research.