Semapimod tetrahydrochloride is primarily used in research settings to study its potential therapeutic effects, particularly in the context of inflammatory and autoimmune diseases. It functions as an inhibitor of cytokine production, which plays a critical role in modulating immune responses. This makes it a candidate for investigating treatments for conditions like rheumatoid arthritis, Crohn's disease, and other inflammatory disorders. Additionally, its ability to suppress tumor necrosis factor-alpha (TNF-α) has sparked interest in exploring its use in cancer therapy, where inflammation is often a contributing factor to disease progression. Researchers are also examining its potential in managing sepsis, as it may help control the excessive immune response associated with this life-threatening condition. Its application extends to preclinical studies aimed at understanding its mechanism of action and optimizing its efficacy for future clinical use.