ERK5-IN-1 is primarily used in research settings to study the role of the ERK5 signaling pathway in various cellular processes. It is a selective inhibitor of ERK5 (extracellular signal-regulated kinase 5), making it a valuable tool for investigating the biological functions of this kinase. Researchers utilize ERK5-IN-1 to explore its impact on cell proliferation, differentiation, and survival, particularly in the context of cancer and inflammatory diseases. By inhibiting ERK5, it helps to elucidate the pathway's involvement in tumor growth and metastasis, providing insights for potential therapeutic strategies. Additionally, it is employed in studies focused on cardiovascular diseases, where ERK5 signaling is known to play a critical role in endothelial cell function and vascular development. Its application extends to understanding the molecular mechanisms underlying neurodegenerative disorders, as ERK5 is implicated in neuronal survival and stress responses. Overall, ERK5-IN-1 serves as a critical compound for advancing knowledge in cellular signaling and disease pathology.