ERK5-IN-1 is primarily used in research settings to study the role of the ERK5 signaling pathway in various biological processes. It is a selective inhibitor of extracellular signal-regulated kinase 5 (ERK5), making it a valuable tool for investigating cellular functions such as proliferation, differentiation, and survival. Researchers utilize this compound to explore its potential in modulating diseases where ERK5 is implicated, including cancer, cardiovascular disorders, and inflammatory conditions. By inhibiting ERK5, it helps in understanding the pathway's contribution to tumor growth and metastasis, providing insights for developing targeted therapies. Additionally, it is employed in preclinical studies to assess its efficacy and safety as a potential therapeutic agent.