Used as a crosslinking agent in bioconjugation, particularly for linking proteins and peptides. Its two maleimide groups react selectively with thiol groups (-SH) under mild conditions, forming stable thioether bonds. Commonly applied in the preparation of antibody-drug conjugates, protein labeling, and immobilization of biomolecules on surfaces. Also utilized in polymer chemistry to modify and crosslink thiol-containing polymers, enhancing mechanical and thermal properties. Preferred in applications requiring controlled, site-specific coupling due to its homobifunctional structure and relatively short spacer arm.