Used primarily as a crosslinking agent in bioconjugation, enabling the stable linkage of peptides, proteins, and other biomolecules. Its dual maleimide groups react selectively with thiol groups (-SH) in cysteine residues, forming covalent bonds under mild conditions. Commonly applied in the development of antibody-drug conjugates (ADCs), protein labeling, and immobilization of enzymes on surfaces. Also utilized in hydrogel formation for tissue engineering and drug delivery systems due to its ability to bridge thiolated polymers. Stable in organic solvents and effective at physiological pH, making it suitable for both in vitro and in vivo applications.