Monodispersed magnetite microspheres are widely used in biomedical applications, particularly in magnetic resonance imaging (MRI) as contrast agents to enhance image clarity. They are also employed in targeted drug delivery systems, where their magnetic properties allow precise localization of therapeutic agents to specific tissues or organs. In environmental science, these microspheres are utilized for water purification, effectively removing heavy metals and contaminants through magnetic separation. Additionally, they play a significant role in bioseparation processes, enabling the efficient isolation of biomolecules such as proteins and DNA. Their uniform size and magnetic responsiveness make them valuable in research and industrial applications, including catalysis and the development of advanced materials.