Monodispersed magnetite microspheres are widely used in biomedical applications due to their magnetic properties and uniform size. They are employed in magnetic resonance imaging (MRI) as contrast agents to enhance image clarity, particularly in diagnosing tumors and other abnormalities. These microspheres are also utilized in targeted drug delivery systems, where their magnetic nature allows precise control over the release of therapeutic agents to specific areas of the body, minimizing side effects. In addition, they play a crucial role in magnetic separation techniques, enabling the efficient isolation of cells, proteins, or DNA from complex mixtures in research and diagnostics. Furthermore, they are used in hyperthermia treatment for cancer, where they generate heat under an alternating magnetic field to destroy cancer cells selectively. Their uniformity and stability make them valuable in various industrial applications, including wastewater treatment for the removal of heavy metals and other contaminants.