Phospho-Protein Extraction Buffer is widely used in molecular biology and biochemistry research for the efficient extraction of phosphorylated proteins from cells and tissues. It is particularly valuable in studies focusing on signal transduction pathways, where the phosphorylation status of proteins plays a critical role in cellular processes. This buffer helps maintain the integrity of phosphoproteins during extraction, preventing dephosphorylation and degradation, which is essential for accurate analysis. Researchers often use it in conjunction with techniques like Western blotting, immunoprecipitation, and mass spectrometry to study protein phosphorylation dynamics. Its application is crucial in cancer research, drug development, and understanding cellular responses to external stimuli, as it enables the detection and quantification of phosphorylated proteins involved in disease mechanisms and therapeutic targets.