MES Lysis Buffer with NP-40 is widely used in biochemical and molecular biology research for cell lysis and protein extraction. Its primary application is in the isolation of proteins from cells or tissues while maintaining their native structure and function. The buffer’s combination of MES (a pH-stabilizing agent) and NP-40 (a non-ionic detergent) ensures efficient disruption of cell membranes without denaturing proteins, making it ideal for downstream applications such as Western blotting, immunoprecipitation, and enzyme activity assays. It is particularly useful for extracting membrane-associated proteins due to NP-40’s ability to solubilize lipid bilayers. Additionally, its mild nature minimizes interference with protein-protein interactions, making it suitable for studying protein complexes. This buffer is commonly employed in studies involving cell signaling pathways, protein localization, and proteomics research.