MES Lysis Buffer with NP-40 is widely used in molecular biology and biochemistry for cell lysis and protein extraction. Its primary application is to disrupt cell membranes while maintaining the integrity of proteins and other cellular components. The buffer is particularly effective for isolating proteins from mammalian cells, as NP-40, a non-ionic detergent, solubilizes membrane proteins without denaturing them. This makes it a preferred choice for preparing samples for techniques like Western blotting, immunoprecipitation, and protein purification. Additionally, the MES buffer component provides a stable pH environment, ensuring optimal conditions for downstream assays. Its versatility and effectiveness in preserving protein function make it a staple in laboratories focused on protein analysis and cellular research.