PIPES lysis buffer with Triton is widely used in molecular biology and biochemistry for cell lysis and protein extraction. Its balanced pH and mild detergent properties make it ideal for disrupting cell membranes while preserving protein integrity. This buffer is commonly employed in studies involving membrane proteins, as Triton X-100 effectively solubilizes lipid bilayers without denaturing proteins. It is also utilized in immunoprecipitation and co-immunoprecipitation assays to maintain protein-protein interactions. Additionally, the buffer is suitable for preparing cell extracts for downstream applications such as gel electrophoresis, western blotting, and enzymatic assays. Its compatibility with various biochemical techniques makes it a versatile tool in research laboratories.