MES Lysis Buffer with Triton is widely used in molecular biology and biochemistry for cell lysis and protein extraction. Its primary application is in breaking down cell membranes to release intracellular components, such as proteins, nucleic acids, and organelles, while maintaining their stability and functionality. The buffer is particularly effective for solubilizing membrane proteins due to the presence of Triton, a non-ionic detergent that disrupts lipid bilayers without denaturing proteins. It is commonly employed in Western blotting, immunoprecipitation, and other protein analysis techniques to ensure efficient extraction and preservation of target proteins. Additionally, its compatibility with downstream applications, such as electrophoresis and mass spectrometry, makes it a versatile choice for researchers studying protein interactions, signaling pathways, and cellular processes.