HEPES Lysis Buffer with Triton is widely used in biological research for cell lysis and protein extraction. Its primary application is in the preparation of cell lysates for downstream analysis, such as Western blotting, immunoprecipitation, and enzyme activity assays. The buffer's HEPES component maintains a stable pH, which is crucial for preserving protein integrity during the lysis process. Triton X-100, a non-ionic detergent, effectively solubilizes cell membranes, releasing cellular contents while minimizing protein denaturation. This buffer is particularly useful for studying membrane-bound proteins and signaling pathways, as it ensures efficient extraction without disrupting protein-protein interactions. Additionally, it is compatible with various biochemical and molecular biology techniques, making it a versatile tool in laboratory workflows.