PIPES Lysis Buffer with NP-40 is widely used in molecular biology and biochemistry for cell lysis and protein extraction. Its mild and stable pH range makes it suitable for maintaining protein integrity during the extraction process. The inclusion of NP-40, a non-ionic detergent, helps in solubilizing membrane proteins while preserving their native structure and function. This buffer is particularly effective for isolating proteins from mammalian cells, as it efficiently disrupts cell membranes without denaturing proteins. It is commonly employed in immunoprecipitation, co-immunoprecipitation, and other protein-protein interaction studies. Additionally, it is used in preparing cell lysates for downstream applications such as Western blotting, enzyme activity assays, and chromatography. Its compatibility with various biochemical techniques makes it a versatile tool in protein research.