PIPES Lysis Buffer with NP-40 is widely used in molecular biology and biochemistry for cell lysis and protein extraction. It is particularly effective in maintaining the stability and functionality of proteins during the extraction process. The buffer is often employed in studies involving membrane proteins, as NP-40, a non-ionic detergent, helps solubilize membrane components without denaturing proteins. This makes it suitable for preparing cell lysates for downstream applications such as Western blotting, immunoprecipitation, and enzyme activity assays. Additionally, the buffer's compatibility with various biochemical techniques ensures reliable results in protein analysis and purification workflows.