NP40 Lysis Buffer with High Salt is widely used in molecular biology and biochemistry for the efficient extraction of proteins from cells and tissues. Its high salt concentration helps to disrupt protein-protein interactions, making it particularly effective for solubilizing membrane-bound proteins and nuclear proteins. This buffer is commonly employed in immunoprecipitation and co-immunoprecipitation assays, where it aids in maintaining protein stability and integrity during the extraction process. Additionally, it is utilized in the preparation of cellular lysates for downstream applications such as Western blotting, ELISA, and protein purification. The inclusion of NP40, a nonionic detergent, ensures the gentle disruption of cell membranes while preserving the native conformation of proteins, making it a versatile tool in protein research.