Cell Lysis Buffer is primarily used in single-cell gel electrophoresis, commonly known as the comet assay, to break open cell membranes and release cellular contents for analysis. This buffer plays a critical role in facilitating the detection of DNA damage at the individual cell level. By lysing cells, it allows for the separation of DNA fragments during electrophoresis, enabling researchers to assess the extent of DNA strand breaks, alkali-labile sites, and cross-linking. The buffer's formulation ensures optimal conditions for maintaining DNA integrity while exposing damage, making it a key component in genotoxicity testing, environmental monitoring, and cancer research. Its application is essential for studying the effects of mutagens, radiation, and other DNA-damaging agents, providing valuable insights into cellular health and genetic stability.