Red Blood Cell Lysis Buffer is widely used in biological and medical research to selectively lyse red blood cells (RBCs) while preserving the integrity of other cell types, such as white blood cells (WBCs) or nucleated cells. This is particularly useful in applications like isolating peripheral blood mononuclear cells (PBMCs) from whole blood samples. It is commonly employed in flow cytometry, immunophenotyping, and cell culture preparation to remove RBCs that might interfere with downstream analyses. The buffer is also utilized in molecular biology experiments, such as DNA or RNA extraction, where the elimination of RBCs is necessary to obtain pure samples. Its application extends to clinical diagnostics, where it aids in the study of hematological disorders or infections by isolating specific cell populations for further examination. The buffer's ability to efficiently remove RBCs without damaging other cells makes it a critical tool in both research and diagnostic workflows.