HEPES Protein Extraction Buffer is widely used in biochemical and molecular biology research for the efficient extraction of proteins from various biological samples. Its primary application lies in maintaining a stable pH environment during the extraction process, which is crucial for preserving protein structure and function. This buffer is particularly useful in experiments involving cell lysis, where it helps to solubilize proteins while minimizing denaturation or degradation. It is also employed in protein purification protocols, including chromatography and electrophoresis, to ensure optimal conditions for protein stability. Additionally, HEPES Protein Extraction Buffer is compatible with a wide range of downstream applications, such as Western blotting, enzyme assays, and protein-protein interaction studies, making it a versatile tool in protein research. Its ability to maintain pH in the physiological range (typically around 7.0-8.0) makes it suitable for use with sensitive proteins and enzymes that require precise pH control.