HEPES-Triton Protein Extraction Buffer is widely used in biological research for extracting proteins from cells and tissues. Its primary application is in the preparation of protein samples for downstream analysis, such as Western blotting, immunoprecipitation, or enzymatic assays. The buffer's combination of HEPES, a pH-stabilizing agent, and Triton, a non-ionic detergent, effectively lyses cell membranes while maintaining protein integrity and activity. This makes it particularly useful for studies requiring the extraction of membrane-bound proteins or proteins sensitive to harsh conditions. Additionally, it is compatible with various cell types and tissues, ensuring consistent and reliable protein extraction for experimental reproducibility.