Used for efficient lysis of yeast cells and extraction of soluble proteins for downstream applications such as enzyme assays, western blotting, and protein purification. Maintains protein stability and activity by including protease inhibitors and buffering agents. Suitable for both Saccharomyces cerevisiae and other yeast species in research involving proteomics, metabolic studies, and recombinant protein expression. Can be used in high-throughput screening and small-scale lysates without mechanical disruption.