RIPA Lysis Buffer (Weak) is widely used in molecular biology and biochemistry for the extraction and solubilization of proteins from cells and tissues. Its mild formulation makes it particularly suitable for applications where preserving protein-protein interactions or maintaining protein activity is crucial. It is commonly employed in immunoprecipitation experiments, as it effectively lyses cells while minimizing the disruption of protein complexes. Additionally, it is used in Western blotting and other protein analysis techniques to prepare samples for electrophoresis, ensuring that proteins remain intact and functional. Its compatibility with downstream assays, such as enzyme activity measurements or protein quantification, makes it a versatile tool in research laboratories.