DL-Mevalonolactone is widely used in biochemical research as a precursor in the mevalonate pathway, which plays a key role in cholesterol and isoprenoid biosynthesis. It serves as a substrate in enzyme assays involving mevalonate kinase and other downstream enzymes in sterol metabolism. Due to its role in this metabolic pathway, it is applied in studies related to lipid regulation, cell signaling, and the development of drugs targeting hypercholesterolemia. It is also used in cell culture experiments to modulate intracellular levels of isoprenoids, helping researchers investigate protein prenylation and its effects on cell proliferation and apoptosis. Additionally, DL-Mevalonolactone finds use in metabolic labeling and tracer studies to track biosynthetic routes in living organisms.