OH-C-Chol is primarily utilized in the field of biochemistry and medical research, particularly in studies involving cholesterol metabolism and lipid biology. It serves as a valuable tool for investigating the mechanisms of cholesterol absorption, transport, and storage within cells. Researchers often employ OH-C-Chol to label or trace cholesterol pathways, enabling a deeper understanding of its role in cellular processes and diseases such as atherosclerosis. Additionally, it is used in the development of diagnostic assays and therapeutic strategies targeting cholesterol-related disorders. Its application extends to the study of membrane dynamics and lipid rafts, where it helps elucidate the structural and functional aspects of cellular membranes.