20-hydroxy PGF2α plays a significant role in reproductive physiology, particularly in the regulation of luteal function. It is involved in the process of luteolysis, which is the degradation of the corpus luteum, leading to the cessation of progesterone production. This is crucial for the menstrual cycle and pregnancy maintenance in mammals. In veterinary medicine, it is used to manage reproductive cycles in livestock, aiding in synchronization of estrus and improving breeding efficiency. Additionally, it is studied for its potential therapeutic applications in managing certain reproductive disorders in humans, such as endometriosis and abnormal uterine bleeding. Its role in inflammatory processes and smooth muscle contraction also makes it a subject of interest in research related to cardiovascular and respiratory diseases.