ACAT-IN-8 is primarily used in biochemical research to study the function and inhibition of acyl-CoA:cholesterol acyltransferase (ACAT) enzymes. These enzymes play a critical role in cholesterol esterification, a process essential for cellular cholesterol homeostasis. By inhibiting ACAT, researchers can investigate its impact on cholesterol metabolism, which is relevant to understanding and potentially treating atherosclerosis and other cholesterol-related disorders. ACAT-IN-8 serves as a valuable tool in preclinical studies to explore therapeutic strategies for managing cardiovascular diseases and metabolic conditions. Its application is mostly confined to laboratory settings for experimental purposes.