ACAT-IN-7 is primarily utilized in research settings to study the inhibition of acyl-CoA:cholesterol acyltransferase (ACAT) enzymes. These enzymes play a critical role in cholesterol esterification, a process essential for cellular cholesterol homeostasis. By inhibiting ACAT, this compound helps researchers investigate mechanisms related to cholesterol metabolism and its impact on diseases such as atherosclerosis, hypercholesterolemia, and other lipid-related disorders. It is also used in preclinical studies to evaluate potential therapeutic strategies for managing these conditions. Additionally, ACAT-IN-7 aids in understanding the role of ACAT in lipid droplet formation and foam cell development, which are key factors in cardiovascular disease progression. Its application extends to exploring the broader implications of ACAT inhibition in metabolic and inflammatory pathways.