ACAT-IN-6 is primarily utilized in research settings to study the inhibition of acyl-CoA:cholesterol acyltransferase (ACAT) enzymes. Its application is significant in understanding cholesterol metabolism and its role in diseases such as atherosclerosis. Researchers use ACAT-IN-6 to investigate how blocking ACAT activity can reduce the accumulation of cholesterol esters in cells, potentially leading to therapeutic strategies for managing hypercholesterolemia and related cardiovascular conditions. Additionally, it serves as a tool in preclinical studies to evaluate the efficacy and mechanisms of ACAT inhibitors in lipid regulation and plaque formation.