ACAT-IN-5 is primarily utilized in biochemical research to study the mechanisms of cholesterol metabolism. It serves as an inhibitor of acyl-CoA:cholesterol acyltransferase (ACAT), an enzyme involved in the esterification of cholesterol. By inhibiting ACAT, this compound helps researchers explore the role of cholesterol esterification in cellular processes, particularly in the context of atherosclerosis and lipid storage diseases. Its application extends to preclinical studies aimed at developing therapeutic strategies for managing hypercholesterolemia and related cardiovascular disorders. Additionally, ACAT-IN-5 is used in vitro to investigate lipid droplet formation and its implications in metabolic disorders.