ACAT-IN-4 is primarily utilized in biochemical research to study the mechanisms of cholesterol metabolism. It acts as an inhibitor of acyl-coenzyme A: cholesterol acyltransferase (ACAT), an enzyme involved in the esterification of cholesterol. By inhibiting ACAT, it helps researchers understand the role of cholesterol esterification in cellular processes and its implications in diseases such as atherosclerosis. This compound is particularly valuable in experimental settings aimed at exploring potential therapeutic strategies for managing cholesterol-related disorders. Its application extends to in vitro studies where it is used to modulate cholesterol storage and transport within cells, providing insights into lipid homeostasis and its impact on cardiovascular health.