ACAT-IN-10 is primarily utilized in biochemical research as a potent inhibitor of acyl-CoA:cholesterol acyltransferase (ACAT), an enzyme involved in cholesterol esterification. Its application is significant in studies focused on understanding cholesterol metabolism and its role in diseases such as atherosclerosis and hypercholesterolemia. Researchers employ ACAT-IN-10 to investigate the potential therapeutic effects of ACAT inhibition in reducing cholesterol absorption and accumulation in cells, which could lead to the development of treatments for cardiovascular diseases. Additionally, it is used in experimental models to explore its impact on lipid homeostasis and its potential as a target for lipid-lowering drugs.