SMS2-IN-1 is primarily utilized in biochemical research to study and modulate the activity of sphingomyelin synthase 2 (SMS2), an enzyme involved in lipid metabolism. It is used as a potent and selective inhibitor of SMS2, enabling researchers to investigate the role of this enzyme in various cellular processes, including lipid signaling, membrane composition, and metabolic regulation.

In the context of disease research, SMS2-IN-1 is employed to explore its potential therapeutic effects in conditions related to lipid dysregulation, such as atherosclerosis, obesity, and non-alcoholic fatty liver disease (NAFLD). By inhibiting SMS2, it helps to understand the enzyme's contribution to lipid accumulation and inflammation, which are key factors in these diseases.

Additionally, SMS2-IN-1 is used in pharmacological studies to assess its efficacy and safety as a potential drug candidate, providing insights into its mechanism of action and therapeutic applications. Its role in modulating lipid pathways makes it a valuable tool for advancing knowledge in metabolic and cardiovascular research.