Protein Kinase A (PKA) from bovine heart is widely used in biochemical research to study signal transduction pathways. It plays a critical role in phosphorylating target proteins, regulating various cellular processes such as metabolism, gene expression, and cell division. Researchers utilize PKA to investigate cAMP-dependent signaling mechanisms, which are essential for understanding hormone action, neural function, and cardiac regulation. It is also employed in enzyme assays to screen for inhibitors or activators, aiding drug discovery efforts targeting diseases like cancer, cardiovascular disorders, and metabolic syndromes. Additionally, PKA serves as a tool in studying protein-protein interactions and post-translational modifications, providing insights into cellular signaling networks.