Used extensively in biochemical research to study enzyme kinetics and cellular energy transfer mechanisms. It serves as a modified ATP analog, enabling the investigation of ATP-dependent processes without the interference of endogenous ATP. Commonly applied in assays to monitor the activity of ATPases, kinases, and other ATP-utilizing enzymes. Its periodate-oxidized structure makes it valuable in crosslinking studies, helping to analyze protein-protein or protein-nucleic acid interactions. Additionally, it is utilized in the development of diagnostic tools and therapeutic agents targeting energy metabolism pathways.