Adenosine 3′,5′-diphosphate disodium salt is widely used in biochemical research as a substrate or inhibitor in enzymatic studies, particularly those involving kinases and other ATP-dependent enzymes. It serves as a valuable tool for investigating metabolic pathways and understanding cellular energy processes. In molecular biology, it is employed in the study of nucleotide interactions and signal transduction mechanisms. Additionally, it finds application in the development of diagnostic assays and pharmaceutical research, where it helps in evaluating drug efficacy and mechanisms of action. Its role in modulating enzyme activity makes it essential for advancing studies in biochemistry and drug discovery.