This chemical is primarily used in biochemical research to study cellular signaling pathways, particularly those involving tyrosine kinases. It serves as a key tool for investigating the mechanisms of autophosphorylation, which is crucial for understanding cell growth, differentiation, and cancer development. Researchers utilize it to analyze the role of phosphorylated tyrosine residues in protein function and their impact on downstream signaling events. It is also employed in assays to evaluate kinase activity and to develop inhibitors targeting specific phosphorylation sites, which can have therapeutic implications in treating diseases like cancer.