Crosstide is primarily used in biochemical research and pharmaceutical development. It serves as a synthetic peptide substrate for kinase assays, particularly in studying the activity and inhibition of protein kinases. This application is crucial for understanding cellular signaling pathways and developing targeted therapies for diseases like cancer. Additionally, Crosstide is utilized in high-throughput screening to identify potential kinase inhibitors, aiding in drug discovery processes. Its stability and specificity make it a valuable tool in enzymology and molecular biology research.