CREBtide is widely used in biochemical research as a substrate to study the activity of protein kinase A (PKA). It is particularly valuable in assays designed to measure PKA phosphorylation, helping researchers understand cellular signaling pathways. This peptide is also utilized in drug discovery to screen for compounds that modulate PKA activity, which is crucial in diseases like cancer, cardiovascular disorders, and neurological conditions. Additionally, CREBtide aids in investigating the cAMP-dependent pathway, providing insights into gene expression regulation and cellular responses to external stimuli. Its application extends to studying the role of PKA in learning and memory processes, making it a key tool in neuroscience research.