A-385358 is primarily utilized in biochemical research as a selective inhibitor targeting specific protein kinases. It is particularly valuable in studies aimed at understanding cellular signaling pathways and the role of kinases in disease mechanisms, such as cancer and inflammation. Researchers use this compound to inhibit kinase activity selectively, allowing them to dissect the contributions of these enzymes to various biological processes. Its application extends to drug discovery efforts, where it serves as a tool compound to validate kinase targets and develop potential therapeutic agents. Additionally, A-385358 is employed in high-throughput screening assays to identify novel inhibitors or modulators of kinase activity, contributing to the development of targeted therapies.