KT5823 is primarily used in biological research as a selective inhibitor of protein kinase G (PKG). It is widely applied in studies to understand the role of PKG in various cellular processes, such as smooth muscle relaxation, platelet aggregation, and neuronal signaling. Researchers utilize KT5823 to investigate the mechanisms underlying cardiovascular diseases, as PKG plays a critical role in vascular tone regulation. Additionally, it is employed in neuroscience to explore PKG's involvement in synaptic plasticity and memory formation. Its inhibitory properties make it a valuable tool for dissecting signaling pathways and identifying potential therapeutic targets in diseases where PKG activity is dysregulated.