KHK-IN-1 hydrochloride is primarily used in research settings to study the role of ketohexokinase (KHK) in metabolic pathways. It is a selective inhibitor of KHK, an enzyme involved in fructose metabolism, making it a valuable tool for investigating conditions related to fructose metabolism disorders, such as obesity, diabetes, and non-alcoholic fatty liver disease (NAFLD). Researchers utilize this compound to explore potential therapeutic strategies targeting fructose metabolism and its impact on metabolic health. Additionally, it aids in understanding the biochemical mechanisms underlying fructose-induced insulin resistance and hepatic steatosis, contributing to the development of novel treatments for metabolic syndromes.