GSK650394 is primarily utilized in research settings as a potent and selective inhibitor of the serum- and glucocorticoid-inducible kinase 1 (SGK1). This compound is particularly valuable in studying the role of SGK1 in various cellular processes, including cell survival, proliferation, and ion transport. Researchers employ GSK650394 to investigate its potential therapeutic applications in conditions such as hypertension, diabetes, and certain types of cancer, where SGK1 activity is implicated. Additionally, it aids in understanding the molecular mechanisms underlying these diseases, providing insights that could lead to the development of novel treatments. Its specificity makes it a crucial tool in biochemical and pharmacological studies focused on SGK1 signaling pathways.