OTS514 hydrochloride is primarily used in research settings to study its effects on cancer cells. It functions as a potent inhibitor of specific kinases involved in cell division, making it valuable for investigating mechanisms of cell cycle regulation and tumor growth inhibition. Researchers utilize this compound to explore potential therapeutic strategies for various cancers, particularly those resistant to conventional treatments. Its ability to target specific pathways offers insights into developing more precise and effective cancer therapies. Additionally, OTS514 hydrochloride is employed in preclinical studies to assess its efficacy, safety, and pharmacokinetics, aiding in the development of novel anticancer drugs.