ILK-IN-3 is primarily utilized in biomedical research as a potent and selective inhibitor of integrin-linked kinase (ILK). It is widely applied in studies focused on understanding the role of ILK in cellular processes such as cell adhesion, migration, and survival. Researchers use ILK-IN-3 to investigate its effects on cancer cell proliferation and metastasis, as ILK is often overexpressed in various cancers. Additionally, it is employed in exploring potential therapeutic strategies for diseases where ILK signaling pathways are dysregulated, including fibrosis and cardiovascular disorders. Its application extends to in vitro and in vivo models to assess its efficacy in modulating ILK-dependent pathways and its potential as a drug candidate.