MELK-8a hydrochloride is primarily utilized in research settings to study its role as a selective inhibitor of maternal embryonic leucine zipper kinase (MELK). It is widely applied in cancer research to investigate the effects of MELK inhibition on tumor growth and cell proliferation. Researchers use it to explore its potential in targeting cancer stem cells, which are often resistant to conventional therapies. Additionally, it serves as a tool compound in molecular biology to understand the signaling pathways and biological functions associated with MELK in various cellular processes. Its application extends to preclinical studies aimed at developing novel therapeutic strategies for cancer treatment.